Recombinant DNA refers to a DNA molecule which contains DNA fragments from more than one source with an established phosphodiester linkage created by DNA Ligase.
Recombinant DNAs have been used to introduce new traits into cells or organisms which do not have such traits previously.
to create a recombinant DNA several factors need to be considered and principles adhered to strictly.
some of the factors includes the
1. source(s) of the new gene of interest
2. Vector to carry gene of interest
3. Is the gene of interest going to be expressed or just for mapping purposes
more information can be obtained from the resources below
Strategies an Principles of creating recombinant DNA
http://www.rpi.edu/dept/chem-eng/Biotech-Environ/Projects00/rdna/rdna.html
http://www.bx.psu.edu/~ross/workmg/Isolat_analyz_genes_Chpt3.htm
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